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M94A0281.TXT
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1994-10-08
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Document 0281
DOCN M94A0281
TI Comparison of nested PCR for detection of DNA in plasma with pp65
leukocytic antigenemia procedure for diagnosis of human cytomegalovirus
infection.
DT 9412
AU Freymuth F; Gennetay E; Petitjean J; Eugene G; Hurault de Ligny B;
Ryckelynck JP; Legoff C; Hazera P; Bazin C; Virus Laboratory, University
Hospital Caen, France.
SO J Clin Microbiol. 1994 Jun;32(6):1614-8. Unique Identifier : AIDSLINE
MED/94358148
AB A nested PCR was used for the detection of human cytomegalovirus (HCMV)
DNA in plasma. The presence of HCMV DNA and its correlation to pp65
leukocytic antigenemia were investigated with 299 blood samples from 45
organ transplant recipients and 63 AIDS patients. Of the 53 samples
positive by nested PCR, 52 (98%) were also positive for leukocytic
antigenemia and 23 had high levels of antigenemia (> 50 positive cells
per 2 x 10(5) leukocytes). Of the 246 samples negative in PCR, only 3
(1.2%) had highly positive antigenemia. For 15 patients having a high
antigenemia level in the course of their disease, consecutive blood
samples were studied and also assessed for viremia in culture. The
extent to which HCMV DNA, detected by PCR, was present in plasma
correlated with increased levels of HCMV leukocytic antigenemia for six
of the eight AIDS patients and for all the organ transplant recipients.
Positivity for HCMV DNA in PCR and for viremia in cell culture was
usually restricted to the highest antigenemia levels. From a total of 69
blood samples, PCR and culture gave positive results, respectively, for
17 of 32 samples (53%) and 14 of 32 samples (43%) from transplant
recipients and for 15 of 37 samples (40%) and 9 of 37 samples (24%) from
AIDS patients. Our findings have shown a strong correlation between high
levels of leukocytic antigenemia and HCMV DNA in plasma. The detection
of HCMV DNA in plasma by this nested PCR can prove HCMV dissemination in
blood, but it lacks the rapidity and simplicity of the leukocytic pp65
antigenemia procedure.
DE AIDS-Related Opportunistic Infections/MICROBIOLOGY Base Sequence
Comparative Study Cytomegalovirus/GENETICS/IMMUNOLOGY/*ISOLATION &
PURIF Cytomegalovirus Infections/*MICROBIOLOGY DNA Primers DNA,
Viral/*BLOOD Human Leukocytes/*MICROBIOLOGY Molecular Sequence Data
Phosphoproteins/*BLOOD *Polymerase Chain Reaction Postoperative
Complications/MICROBIOLOGY Support, Non-U.S. Gov't Transplantation
Viral Matrix Proteins/*BLOOD Viremia/*MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).